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[The Application of Fluo-3 AM in Measurement of Level of Cytoplasmic Calcium in Thrombocytes by Flow Cytofluorometry]

[The Application of Fluo-3 AM in Measurement of Level of Cytoplasmic Calcium in Thrombocytes by Flow Cytofluorometry]

N N Petrischev, L V Vasina, A V Selyutin, S V Chepanov, S A Selkov

Klin Lab Diagn. 2017 Feb;62(2):97-9.

PMID: 30615391

Abstract:

The study was carried out to evaluate possibility of applying technique of thrombin-induced increasing of concentration of Ca in cytoplasm of Fluo-3-colored thrombocytes as an experimental model of studying mechanism of action of anti-thrombocytes medications in vitro. The effect of anti-thrombocyte substances on thrombin-induced increasing of the level of cytoplasmic Ca in thrombocytes was analyzed on example of acetylsalicylic acid. The measurement of concentration of cytoplasmic Ca was implemented using flow cytometry technique with fluorescent probe Fluo-3 AM. It is established that in the given test acetylsalicylic acid inhibits thrombin-induced increasing of cytoplasmic Ca at 0.125-5.0 mk/mol concentrations. This occurrence testifies that in the mechanism of effect of acetylsalicylic acid the suppression of thromboxane path ceases to be a leading one. The proposed methodical approach permits evaluating anti-thrombocite effect of substances according their impact to the level of cytoplasmic Ca in thrombocytes in vitro. However, this approach has a number of limitations preventing wide-spread application of the given technique.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP121714225-B	Fluo 3-AM		121714-22-5	Price

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