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The Autoregulator Aca2 Mediates anti-CRISPR Repression

The Autoregulator Aca2 Mediates anti-CRISPR Repression

Nils Birkholz, Robert D Fagerlund, Leah M Smith, Simon A Jackson, Peter C Fineran

Nucleic Acids Res. 2019 Oct 10;47(18):9658-9665.

PMID: 31428783

Abstract:

CRISPR-Cas systems are widespread bacterial adaptive defence mechanisms that provide protection against bacteriophages. In response, phages have evolved anti-CRISPR proteins that inactivate CRISPR-Cas systems of their hosts, enabling successful infection. Anti-CRISPR genes are frequently found in operons with genes encoding putative transcriptional regulators. The role, if any, of these anti-CRISPR-associated (aca) genes in anti-CRISPR regulation is unclear. Here, we show that Aca2, encoded by the Pectobacterium carotovorum temperate phage ZF40, is an autoregulator that represses the anti-CRISPR-aca2 operon. Aca2 is a helix-turn-helix domain protein that forms a homodimer and interacts with two inverted repeats in the anti-CRISPR promoter. The inverted repeats are similar in sequence but differ in their Aca2 affinity, and we propose that they have evolved to fine-tune, and downregulate, anti-CRISPR production at different stages of the phage life cycle. Specific, high-affinity binding of Aca2 to the first inverted repeat blocks the promoter and induces DNA bending. The second inverted repeat only contributes to repression at high Aca2 concentrations in vivo, and no DNA binding was detectable in vitro. Our investigation reveals the mechanism by which an Aca protein regulates expression of its associated anti-CRISPR.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP110683108	ACA - CAS 110683-10-8		110683-10-8	Price

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