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The CD163 Long-Range Scavenger Receptor Cysteine-Rich Repeat: Expression, Purification and X-ray Crystallographic Characterization

The CD163 Long-Range Scavenger Receptor Cysteine-Rich Repeat: Expression, Purification and X-ray Crystallographic Characterization

Rui Li, Hongfang Ma, Longguang Jiang, Songlin Qiao, Yubao Zhi, Mingdong Huang, Ruiguang Deng, Gaiping Zhang

Acta Crystallogr F Struct Biol Commun. 2018 May 1;74(Pt 5):322-326.

PMID: 29718001

Abstract:

Scavenger receptors (SRs) play critical roles in various physiological and pathological pathways. One of them, CD163, is a multifunctional endocytic receptor and is characterized by a long-range scavenger receptor cysteine-rich (SRCR) repeat. However, the structural and functional details of this long-range SRCR repeat have not yet been elucidated. In this study, the CD163 long-range SRCR repeat was expressed in Drosophila Schneider 2 cells. The recombinant protein was homogeneous after purification by metal-affinity, cation-exchange and size-exclusion chromatography. Single crystals were obtained using 20% PEG 4000, 0.15 M potassium sodium tartrate tetrahydrate pH 8.5 and diffracted to 3.30 Å resolution. As the first view of a long-range SRCR repeat, this work lays the structural basis for a deep understanding of SRs and their multiple functions.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6381595	Potassium sodium tartrate		6381-59-5	Price

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