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[The Cloning Expression, Purification and Activity of Human Angiostatin K (1-3) Gene]

[The Cloning Expression, Purification and Activity of Human Angiostatin K (1-3) Gene]

Yun-Long Wang, Guo-Qiang Wang, Zhi-Tao Li, Xiao-Ya Lu, Yu-Lin Li, Zhen Wang

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Jun;23(3):191-3.

PMID: 20104775

Abstract:

Objective:
To clone the sequence of human Angiostatin cDNA and obtain the protein of recombinant angiostatin for further development.
Methods:
Fresh human liver tissue was used for the extraction of total RNA and amplified the Angiostatin cDNA through RT-PCR method . After the recombinant plasmid pET30a-Angiostatin was constructed and confirmed, it was transduced into Rosetta (DE3). Then the transformation was used for fermentation and induced expression. The protein was identified by Western Blot and purified by Ni-NTA affinity chromatography.
Results:
The sequence of human Angiostatin cDNA was identical with genebank. Angiostatin (K1-3) was expressed and purified. The target protein made up 30% of the total bacterial protein. The purity is above 90%.
Conclusion:
Angiostatin K (1-3) can be reached using fusion vector pET30a. The product have the biological activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413057	Angiostatin K1-3 human			Price

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