Home
Resources
Publications
The Determination of 5-hydroxytryptamine, Related Indolealkylamines and 5-hydroxyindoleacetic Acid in the Bovine Eye by Gas Chromatography-Negative Ion Chemical Ionization Mass Spectrometry

The Determination of 5-hydroxytryptamine, Related Indolealkylamines and 5-hydroxyindoleacetic Acid in the Bovine Eye by Gas Chromatography-Negative Ion Chemical Ionization Mass Spectrometry

S A Best, J M Midgley, W Huang, D G Watson

J Pharm Biomed Anal. Apr-May 1993;11(4-5):323-33.

PMID: 7689343

Abstract:

Methods were developed for the analysis of 5-hydroxytryptamine, related indolealkylamines (tryptamine, melatonin, 5-methoxytryptamine, N-acetyl-5-hydroxytryptamine and 6-hydroxymelatonin) and 5-hydroxyindole-3-acetic acid (5HIAA) in bovine retina, aqueous and vitreous humours. 5-Hydroxytryptamine and related indolealkylamines were extracted and derivatized to form their corresponding pentafluoropropionyl spirocyclic derivatives. 5HIAA was extracted and derivatized to the corresponding pentafluoropropionamide-trifluoroethyl derivative. Identification and quantitation by gas chromatography-negative ion chemical ionization mass spectrometry was made with reference to deuteriated internal standards. 5-Hydroxytryptamine was present in all (n = 34) retinal samples analysed (20.53 +/- 1.64 ng) while N-acetyl-5-hydroxytryptamine was detected in half of the samples of retina (0.06 +/- 0.02 ng). Melatonin (0.15 +/- 0.06 ng) and tryptamine (0.78 +/- 0.34 ng) were detected in only a small number of retinas. 5-Methoxytryptamine was not present in retina. 5-Hydroxytryptamine was also present in aqueous (0.76 +/- 0.17 ng ml-1 and vitreous (0.35 +/- 0.05 ng ml-1' humours from bovine eye. Tryptamine, melatonin, 5-methoxytryptamine and N-acetyl-5-hydroxytryptamine were not detected in bovine aqueous and vitreous humours. 5HIAA was found in both bovine aqueous (2.03 +/- 0.38 ng ml-1) and vitreous (0.65 +/- 0.06 ng ml-1) humours, but its consistent determination in retina was obviated by interference from spurious peaks.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1210839	N-Acetyl-5-hydroxytryptamine		1210-83-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: