The Determination of 5alpha-androstane-3alpha, 17beta-diol in Human Plasma by Radioimmunoassay

P Laband, J A Tresguerres, B P Lisboa, U Volkwein, J Tamm

Acta Endocrinol (Copenh). 1978 Aug;88(4):778-86.

PMID: 581118

Abstract:

Antibodies have been raised in rabbits against 3alpha, 17beta-dihydroxy-5alpha-androstane-6-0-carboxymethyloxime coupled with Cohn's fraction IV-4. The antiserum exhibited significant cross reactions with 5beta-androstane-3alph1, 17beta-diol, 5alpha-dihydrotestosterone, and testosterone. No cross reactions were observed with 5alpha-androstane-3beta,17beta-diol and 5-androstene-3beta,17beta-diol. The methodological criteria for the measurement of 5alpha-androstane-3alpha, 17beta-diol in human plasma were as follows: The specificity was ensured by separating the cross reacting steroids by thin layer chromatography. The intraassay and interassay coefficients of variation were found to be 6.2 and 10.2%, respectively. The sensitivity was 30 pg. The recovery of different amounts of 5alpha-androstane-3alpha,17beta-diol added to human plasma (80, 120, and 200 pg) yielded 91.3, 92.5, and 93.5%, respectively. The following concentrations of 5alpha-androstane-3alpha,17beta-diol have been determined in human plasma (mean +/- SD, ng/dl): Normal males: 18.98 +/- 5.9; normal females: 2.65 +/- 0.27; females with idiopathic hirsutism: 11.9 +/- 6.4; prepubertal children: not detectable.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP68476368-D	Globulins Cohn fraction IV-4 human	$Globulins Cohn fraction IV-4 human$	68476-36-8	Price

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