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The Development of a Biotinylated NAD +-Applied Human Poly(ADP-Ribose) Polymerase 3 (PARP3) Enzymatic Assay

The Development of a Biotinylated NAD +-Applied Human Poly(ADP-Ribose) Polymerase 3 (PARP3) Enzymatic Assay

Ming Ji, Liyuan Wang, Nina Xue, Fangfang Lai, Sen Zhang, Jing Jin, Xiaoguang Chen

SLAS Discov. 2018 Jul;23(6):545-553.

PMID: 29676938

Abstract:

Poly(ADP-ribose) polymerase 3 (PARP3) is an important member of the PARP family and shares high structural similarities with both PARP1 and PARP2. The biological roles of PARP3 are currently under investigation; however, several key reports indicate the integral roles of PARP3 in DNA damage repair, and thus it has been investigated as a novel target in oncology. It is clear that the identification of selective PARP3 inhibitors would further advance the understanding of the biological roles of PARP3. Herein, we describe a modified PARP3 screening assay using biotinylated NAD+ as the specialized substrate. This method relies on the activity of PARP3 to transfer the biotinylated NAD+ onto a histone protein to form ADP-ribosylated histone. The biotin label on this histone protein is then detected and quantifies the intrinsic enzymatic activity of PARP3. We optimized the assay with respect to the histone, NAD+/biotinylated NAD+ mixture, DNA, and PARP3. Our developed screening system was then validated with a reported selective PARP3 inhibitor, ME0328, as well as utilizing five other clinically available PARP1/2 inhibitors. We demonstrated that our assay system was sensitive, efficient, and economical, and we reason that it could be useful for the development of highly selective PARP3 inhibitors in the future.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1445251228	ME0328		1445251-22-8	Price

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