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The Distribution and Function Characterization of the I Type Lysozyme From Apostichopus Japonicus

The Distribution and Function Characterization of the I Type Lysozyme From Apostichopus Japonicus

Cheng Li, Yu Zhao, Tingting Liu, Jun Huang, Qi Zhang, Benkang Liu, Shan Xiao, Han Wang, Bingnan Liu, Jihui Wang, Lina Cong

Fish Shellfish Immunol. 2018 Mar;74:419-425.

PMID: 29079205

Abstract:

Lysozyme is a very important component of the innate immune system and a key molecule that protects against bacterial infection. Sea cucumber i-type lysozyme (Aj-iLys) has been shown to possess multiple functions. In this study, we investigated the function and characterization of Aj-iLys in detail. Spatial distribution analysis showed that Aj-iLys was constitutively expressed in all tested tissues, with dominant expression in the tentacles and respiratory trees. Challenge with the pathogen V. splendidus and LPS stimulation both significantly up-regulated the mRNA expression of Aj-iLys. More importantly, inhibition of Aj-iLys expression by mRNA interference resulted in significant promotion of coelomocyte apoptosis during LPS challenge in vitro. The results indicated that Aj-iLys serves as an important innate immunity factor and plays a key defense role during host-pathogen interactions in sea cucumbers. From the radius of the antimicrobial zone, it was determined that the non-fusion Aj-iLys exerted a remarkable inhibitive effect on tested bacteria in vitro. Functional investigation revealed that Aj-iLys also exhibited isopeptidase activity based on its ability to hydrolyze l-Glutamic acid γ-(4-nitroanilide) in vitro to produce p-NA, which is an analogue of the isopeptide bond. The optimal catalytic conditions for the isopeptidase activity were 37 °C, pH 6.5, and the optimum ionic strength was about 0.050 mol/L.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7300596	L-Glutamic acid γ-(4-nitroanilide)		7300-59-6	Price

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