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The EBI2 Signalling Pathway Plays a Role in Cellular Crosstalk Between Astrocytes and Macrophages

The EBI2 Signalling Pathway Plays a Role in Cellular Crosstalk Between Astrocytes and Macrophages

Aleksandra Rutkowska, Sinead A O'Sullivan, Isabelle Christen, Juan Zhang, Andreas W Sailer, Kumlesh K Dev

Sci Rep. 2016 May 11;6:25520.

PMID: 27166278

Abstract:

EBI2 is a G protein-coupled receptor activated by oxysterol 7α, 25-dihydroxycholesterol (7α25HC) and regulates T cell-dependant antibody response and B cell migration. We recently found EBI2 is expressed in human astrocytes, regulates intracellular signalling and modulates astrocyte migration. Here, we report that LPS treatment of mouse astrocytes alters mRNA levels of EBI2 and oxysterols suggesting that the EBI2 signalling pathway is sensitive to LPS-mediated immune challenge. We also find that conditioned media obtained from LPS-stimulated mouse astrocytes induces macrophage migration, which is inhibited by the EBI2 antagonist NIBR189. These results demonstrate a role for the EBI2 signalling pathway in astrocytes as a sensor for immune challenge and for communication with innate immune cells such as macrophages.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1599432082	NIBR189		1599432-08-2	Price

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