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The Effect of Angoroside C on Pressure Overload-Induced Ventricular Remodeling in Rats

The Effect of Angoroside C on Pressure Overload-Induced Ventricular Remodeling in Rats

Wei Liang Gu, Chang Xun Chen, Xiao Yan Huang, Jian Ping Gao

Phytomedicine. 2015 Jul 15;22(7-8):705-12.

PMID: 26141756

Abstract:

Background:
Our previous study reveals that total rough extract of Radix Scrophulariae has a beneficial effect on ventricular remodeling.
Hypothesis:
After carrying out a series of preliminary experiments, we speculated that angoroside C may be the effective agent.
Study design:
After oral administration, the effect of angoroside C on ventricular remodeling was evaluated by using a pressure-overloaded rat model, some related indexes were detected in vivo.
Methods:
A model of pressure overloaded ventricular remodeling was produced by abdominal aortic constriction (AAC) in rats. The sham-operated rats underwent an identical surgical procedure except for AAC. AAC rats were randomly divided into five groups: model control group, three angoroside C treated groups (7.5, 15 and 30 mg·kg(-1)) and captopril treated group (40 mg·kg(-1)). The rats were orally administered with the corresponding drugs or drinking water for 4 weeks. The levels of blood pressure (BP), left ventricular weight index (LVWI) and heart weight index (HWI) were detected. Myocardium tissue was stained with hematoxylin and eosin or picric acid/sirius red for cardiomyocyte cross-section area or collagen content measurements respectively. The concentrations of angiotensin Ⅱ (Ang Ⅱ), hydroxyproline (Hyp), matrix metalloproteinase 2 (MMP-2), MMP-9 and tissue inhibitor of metalloproteinase-1 (TIMP-1) in myocardium or serum were determined. Real-time RT-PCR was performed to detect the mRNA expressions of endothelin 1 (ET-1), transforming growth factor β1 (TGF-β1).
Results:
Angoroside C significantly reduced the BP, LVWI and HWI, decreased the content of Ang Ⅱ, Hyp, diminished cross sectional area of cardiomyocytes and ameliorated collagen deposition. Additionally, it markedly reduced collagen I and III expressions and regulated matrix metalloproteinase-2, 9 and inhibitors of metalloproteinase expressions. Angoroside C also down regulated the gene expressions of ET-1 and TGF-β1mRNA in myocardium.
Conclusion:
Angoroside C has beneficial effects against ventricular remodeling. The mechanism is likely to be related to decreasing the level of Ang Ⅱ, attenuating the mRNA expressions of ET-1 and TGF-β1.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP115909223	Angoroside C		115909-22-3	Price

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