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The Effect of the Fluorescent Probe, 3,3'-dipropylthiadicarbocyanine Iodide, on the Energy Metabolism of Ehrlich Ascites Tumor Cells

The Effect of the Fluorescent Probe, 3,3'-dipropylthiadicarbocyanine Iodide, on the Energy Metabolism of Ehrlich Ascites Tumor Cells

T C Smith, J T Herlihy, S C Robinson

J Biol Chem. 1981 Feb 10;256(3):1108-10.

PMID: 7451493

Abstract:

The fluorescent dye, 3,3'-dipropylthiadicarbocyanine iodide, is frequently used to estimate the cell membrane potential of small cells. We have tested the effects of this dye on glycolytic rate, O2 consumption, and cellular ATP content in Ehrlich ascites tumor cells. Addition of the dye to steady state cells in the absence of glucose induces a rapid depletion (half-time approximately equal to 5 min) of cellular ATP, which is secondary to a 76% inhibition of O2 consumption. Aerobic glycolysis is stimulated by 76%. Valinomycin produces an additional stimulation of acid production. Our findings indicate that this fluorescent dye alters energy metabolism of Ehrlich ascites tumor cells. The response is consistent with primary inhibition of oxidative phosphorylation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP53213948	3,3′-Dipropylthiadicarbocyanine iodide		53213-94-8	Price

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