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The Effects of (±)-Praeruptorin A on Airway Inflammation, Remodeling and Transforming Growth factor-β1/Smad Signaling Pathway in a Murine Model of Allergic Asthma

The Effects of (±)-Praeruptorin A on Airway Inflammation, Remodeling and Transforming Growth factor-β1/Smad Signaling Pathway in a Murine Model of Allergic Asthma

You-yi Xiong, Jun-song Wang, Fei-hua Wu, Juan Li, Ling-yi Kong

Int Immunopharmacol. 2012 Dec;14(4):392-400.

PMID: 22974581

Abstract:

(±)-Praeruptorin A (PA) is a pair of coumarin enantiomers isolated from the root of Peucedanum praeruptorum Dunn (PPD), a common Chinese herbal medicine for the treatment of asthma. Considering its anti-inflammatory, anti-contractile and anti-hyperplasia activities, the effects of PA on airway inflammation and airway remodeling were investigated using a murine model of chronic asthma. Ovalbumin-sensitized BALB/c mice were challenged with ovalbumin to induce asthma every other day on eight successive weeks. PA was administered intragastrically before every ovalbumin challenge. Airway responsiveness was evaluated by a lung function analysis system 48 h after the last ovalbumin challenge. The total and differential leukocytes in bronchoalveolar lavage fluid (BALF) were counted using a hemocytometer and Diff-Quick-stained smears. Lung tissue samples were used for hematoxylin and eosin, periodic acid Schiff, Masson's trichrome and α-SMA immunohistochemistry staining. Levels of cytokines in BALF, immunoglobulin (Ig) E in serum as well as expression of TGF-β1 and Smad proteins in lung tissue were measured by enzyme-linked immunosorbent assay, immunohistochemistry or western blot analysis. Compared with the model group, PA suppressed airway inflammation, airway hyperresponsive and remodeling, reduced levels of IL-4 and IL-13 in BALF, and IgE in serum, inhibited expression of TGF-β1 and pSmad2/3, up-regulated the expression of Smad7 in lung tissue, and also increased the levels of INF-γ in BALF. These results suggested that PA significantly suppressed airway inflammation and airway remodeling induced by ovalbumin challenge, and is a potential candidate for the treatment of asthma.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP73069257	(±)-Praeruptorin A		73069-25-7	Price

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