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The Identification and Characterization of Chitotriosidase Activity in Pancreatin From Porcine Pancreas

The Identification and Characterization of Chitotriosidase Activity in Pancreatin From Porcine Pancreas

Chia-Rui Shen, Chao-Lin Liu, Hsiao-Ping Lee, Jeen-Kuan Chen

Molecules. 2013 Mar 4;18(3):2978-87.

PMID: 23459306

Abstract:

The versatile oligosaccharide biopolymers, chitin and chitosan, are typically produced using enzymatic processes. However, these processes are usually costly because chitinases and chitosanases are available in limited quantities. Fortunately, a number of commercial enzymes can hydrolyze chitin and chitosan to produce long chain chitin or chitosan oligosaccharides. Here, a platform to screen for enzymes with chitinase and chitosanase activities using a single gel with glycol chitin or glycol chitosan as a substrate was applied. SDS-resistant chitinase and chitosanase activities were observed for pancreatin. Its chitotriosidase had an optimal hydrolysis pH of 4 in the substrate specificity assay. This activity was thermally unstable, but independent of 2-mercaptoethanol. This is the first time a chitotriosidase has been identified in the hog. This finding suggests that oligochitosaccharides can be mass-produced inexpensively using pancreatin.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP8049476-C	Pancreatin from porcine pancreas		8049-47-6	Price

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