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The Improvement of Chloroperoxidase Activities in the Presence of Ammonium Salts and Cationic Surfactants

The Improvement of Chloroperoxidase Activities in the Presence of Ammonium Salts and Cationic Surfactants

Xuejiao Ru, Jinyue Wu, Huan Guo, Yucheng Jiang, Mancheng Hu, Quanguo Zhai, Shuni Li

Biotechnol Prog. Jul-Aug 2010;26(4):1024-8.

PMID: 20205260

Abstract:

The catalytic activities of chloroperoxidase (CPO) including halogenation, oxidation, and peroxidation were investigated in the presence of ammonium salts: tetramethylammonium bromide (TMABr), tetraethylammonium bromide (TEABr), tetrapropylammonium bromide (TPABr), tetrabutylammonium bromide (TBABr), and cationic surfactants: dodecyltrimethylammonium bromide (DTABr) and cetyltrimethylammonium bromide (CTABr). All the mentioned activities were promoted in most cases. The highest modified activity (ABTS peroxidation) was 18.16 times higher in the presence of TMABr than that in pure buffer. The activity enhancement was strongly dependent on the concentration and the hydrophobic chain length of additives, and the structure of substrates. The kinetic parameters showed that the activation was mainly attributed to an increase in k(cat) due probably to a catalytically favorable conformation of CPO induced by the additives. Moreover, a lower K(m) and higher ratio of k(cat)/K(m) (specificity constant) was obtained, indicating that both the affinity and specificity of CPO to substrates were improved in the presence of additives.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP64200	Tetramethylammonium bromide		64-20-0	Price

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