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[The Influence of Erythropoietin (Epo) on Intercellular Adhesion molecule-1 (ICAM-1, CD54) and Platelet-Endothelial Cell Adhesion molecule-1 (PECAM-1, CD31) Expression on Human Umbilical Vein Endothelial Cells (HUVEC) Induced by Tumor Necrosis Factor-Alpha (TNF-alpha)]

[The Influence of Erythropoietin (Epo) on Intercellular Adhesion molecule-1 (ICAM-1, CD54) and Platelet-Endothelial Cell Adhesion molecule-1 (PECAM-1, CD31) Expression on Human Umbilical Vein Endothelial Cells (HUVEC) Induced by Tumor Necrosis Factor-Alpha (TNF-alpha)]

Marek Szczepański, Monika Kamianowska, Krzysztof Wnuczko, Mirosława Pietruczuk, Sławomir Wołczyński

Wiad Lek. 2007;60(1-2):22-7.

PMID: 17607964

Abstract:

The latest research proved that erythropoietin (Epo), a sensitive to hypoxia physiological erythropoiesis regulator, used successfully to treat anemia, displays cytoprotective, angiogenic, anti-inflammatory, anti-oxidative and anti-apoptotic properties. The aim of the study was to examine the influence of Epo on intercellular adhesion molecule-1 (ICAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM--1) expression on human umbilical vein endothelial cells (HUVEC) induced by tumor necrosis factor-a (TNF-alpha).
Material and methods:
Human umbilical vein endothelial cells were cultured in a standard medium (M 199). The experiment was performed five times on the forth passage of HUVEC culture. For stimulation TNF-alpha was used in concentrations: 10, 20, 40 ng/ml (6 hours) and Epo in concentrations: 10, 20, 40 IU/ml (24 hours). The expression level of ICAM-1 and PECAM-1 on HUVEC were quantified by flow cytometry.
Results:
The Incubation of HUVEC with TNF-a significantly increased the ICAM-1 and PECAM-I expression. The cultures pretreated with Epo reduced ICAM-1 and PECAM-I expression induced by TNF-a from 70.0 +/- 3.94% to 59.3 +/- 0.60% and from 83.4 +/- 2.27% to 57.7 +/- 0.66% respectively for ICAM-1 and PECAM-1.
Conclusions:
Erythropoietin statistically significantly decreases ICAM-1 and PECAM-1 expression on HUVEC stimulated by TNF-alpha.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414465	PECAM-I human			Price

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