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The Phorbol 12-myristate-13-acetate Differentiation Protocol Is Critical to the Interaction of THP-1 Macrophages With Salmonella Typhimurium

The Phorbol 12-myristate-13-acetate Differentiation Protocol Is Critical to the Interaction of THP-1 Macrophages With Salmonella Typhimurium

Tregei Starr, Timothy J Bauler, Preeti Malik-Kale, Olivia Steele-Mortimer

PLoS One. 2018 Mar 14;13(3):e0193601.

PMID: 29538403

Abstract:

THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and compare the ability of THP-1 macrophages to interact with the facultative intracellular pathogen Salmonella enterica serovar Typhimurium. The results show that THP-1 macrophages differentiated in high concentrations of PMA rapidly died following infection whereas those differentiated in low concentrations of PMA survived and were able to control the intracellular bacteria similar to primary human macrophages.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP16561298-A	Phorbol 12-myristate 13-acetate		16561-29-8	Price

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