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The Photostability of the Commonly Used biotin-4-fluorescein Probe

The Photostability of the Commonly Used biotin-4-fluorescein Probe

Richard A Haack, Kerry M Swift, Qiaoqiao Ruan, Richard J Himmelsbach, Sergey Y Tetin

Anal Biochem. 2017 Aug 15;531:78-82.

PMID: 28545867

Abstract:

Biotin-4-fluorescein (B4F) is a commonly used fluorescent probe for studying biotin-(strept)avidin interactions. During a characterization study of an anti-biotin antibody, using B4F as the probe, we noticed a discrepancy in the expected and experimentally determined number of biotin binding sites. Analytical testing showed that the biotin moiety in the probe undergoes a photosensitized oxidation to produce a mixture of biotin sulfoxides which has the potential to impact the quantitation of binding sites using this fluorescent probe.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR424181	Biotin-4-Fluorescein			Price

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