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The Physico-Chemical Characterization of Casein-Modified Surfaces and Their Influence on the Adhesion of Spores From a Geobacillus Species

The Physico-Chemical Characterization of Casein-Modified Surfaces and Their Influence on the Adhesion of Spores From a Geobacillus Species

Jingtian Han, R Brent Seale, Patrick Silcock, A James McQuillan, Phil J Bremer

Biofouling. 2011 May;27(5):459-66.

PMID: 21598124

Abstract:

To gain a better understanding of the factors influencing spore adhesion in dairy manufacturing plants, casein-modified glass surfaces were prepared and characterized and their effect on the adhesion kinetics of spores from a Geobacillus sp., isolated from a dairy manufacturing plant (DMP) was assessed using a flow chamber. Surfaces were produced by initially silanizing glass using (3-glycidyloxypropyl) trimethoxysilane (GPS) or (3-aminopropyl) triethoxysilane to form epoxy-functionalized (G-GPS) or amino-functionalized glass (G-NH(2)) substrata. Casein was grafted to the G-GPS directly by its primary amino groups (G-GPS-casein) or to G-NH(2) by employing glutaraldehyde as a linking agent (G-NH(2)-glutar-casein). The surfaces were characterised using streaming potential measurements, contact angle goniometry, infrared spectroscopy and scanning electron microscopy. The attachment rate of spores suspended in 0.1 M KCl at pH 6.8, was highest on the positively charged (+14 mV) G-NH(2) surface (333 spores cm(-2) s(-1)) compared to the negatively charged glass (-22 mV), G-GPS (-20 mV) or G-GPS-casein (-21 mV) surfaces (162, 17 or 6 spores cm(-2) s(-1) respectively). Whilst there was a clear decrease in attachment rate to negatively charged casein-modified surfaces compared to the positively charged amine surface, there was no clear relationship between surface hydrophobicity and spore attachment rate.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2602348	(3-Glycidyloxypropyl)triethoxysilane		2602-34-8	Price

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