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The Poly(C) Motif in the Proximal Promoter Region of the D Site-Binding Protein Gene ( Dbp) Drives Its High-Amplitude Oscillation

The Poly(C) Motif in the Proximal Promoter Region of the D Site-Binding Protein Gene ( Dbp) Drives Its High-Amplitude Oscillation

Paul Kwangho Kwon, Hyo-Min Kim, Sung Wook Kim, Byunghee Kang, Hee Yi, Hyun-Ok Ku, Tae-Young Roh, Kyong-Tai Kim

Mol Cell Biol. 2019 Jul 29;39(16):e00101-19.

PMID: 31160492

Abstract:

The D site-binding protein (Dbp) supports the rhythmic transcription of downstream genes, in part by displaying high-amplitude cycling of its own transcripts compared to other circadian-clock genes. However, the underlying mechanism remains elusive. Here, we demonstrated that the poly(C) motif within the Dbp proximal promoter, in addition to an E-box element, provoked transcriptional activation. Furthermore, we generated a cell line with poly(C) deleted to demonstrate the endogenous effect of the poly(C) motif within the Dbp promoter. We investigated whether RNA polymerase 2 (Pol2) recruitment on the Dbp promoter was decreased in the cell line with poly(C) deleted. Next, assay for transposase-accessible chromatin (ATAC)-quantitative PCR (qPCR) showed that the poly(C) motif induced greater chromatin accessibility within the region of the Dbp promoter. Finally, we determined that the oscillation amplitude of endogenous Dbp mRNA of the cell line with poly(C) deleted was decreased, which affected the oscillation of other clock genes that are controlled by Dbp Taken together, our results provide new insights into the function of the poly(C) motif as a novel cis-acting element of Dbp, along with its significance in the regulation of circadian rhythms.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP26936403	poly(C)		26936-40-3	Price

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