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The Presence of Concanavalin A and Canatoxin in Canavalia Ensiformis DC Tissue Culture

A Sato, G B Barcellos, E C Riedel, J A Carneiro, C R Carlini, M A Esquibel

Plant Cell Rep. 1993 Feb;12(4):233-6.

PMID: 24197027

Abstract:

Isolated embryos, cotyledons and embryos plusa fragment of cotyledon from seeds of Canavalia ensiformis (jack bean) were cultured in vitro. Concanavalin A and canatoxin cross-reactive material were detected by double immunodiffusion tests. Canatoxin was detectable until 30 days in cultures of embryos, embryos plus cotyledons and hypocotyls. Concanavalin A was also present in all cultures being detected until 90 days in cultures treated with 6-benzylaminopurine. No concanavalin A was detected in root cultures. Concanavalin A was present in cell suspensions until 45 days of culture; the culture medium contained neither concanavalin A nor canatoxin. Tissue cultures thus can produce Con A and CNTX and will be an important research tool for studying the biosynthesis of such substances.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP11028710 Concanavalin A from Canavalia ensiformis (Jack bean) Concanavalin A from Canavalia ensiformis (Jack bean) 11028-71-0 Price
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