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The Production and Characterisation of Dinitrocarbanilide Antibodies Raised Using Antigen Mimics

The Production and Characterisation of Dinitrocarbanilide Antibodies Raised Using Antigen Mimics

Lisa Connolly, Terence L Fodey, Steven R H Crooks, Philippe Delahaut, Christopher T Elliott

J Immunol Methods. 2002 Jun 1;264(1-2):45-51.

PMID: 12191508

Abstract:

Polyclonal antibodies were produced to detect the coccidiostat nicarbazin. Due to structural constraints of the active component of nicarbazin, dinitrocarbanilide (DNC), three different compounds that shared a common substructure with DNC were used as antigen mimics. The compounds (N-succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide (SAN), L-glutamic acid gamma-(p-nitroanilide) (GAN) and p-nitrosuccinanilic acid (NSA)) were conjugated to a carrier protein and used in the immunisation of rabbits. Five different polyclonal sera were produced and consequently characterised. The antibodies exhibited an IC(50) range of 2.3-7.6 ng/ml using a competitive ELISA procedure. Serum from one rabbit, R555, exhibited an IC(50) of 2.9 ng/ml for DNC and cross-reactivity studies showed that this serum was specific for DNC and did not cross-react with other coccidiostats such as halofuginone, toltrazuril or ronidazole.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP67953086	L-Glutamic acid γ-(p-nitroanilide) hydrochloride		67953-08-6	Price

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