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The Pyrimidine Nucleoside Phosphorylase of Mycoplasma Hyorhinis and How It May Affect Nucleoside-Based Therapy

The Pyrimidine Nucleoside Phosphorylase of Mycoplasma Hyorhinis and How It May Affect Nucleoside-Based Therapy

J Vande Voorde, S Liekens, F Gago, J Balzarini

Nucleosides Nucleotides Nucleic Acids. 2014;33(4-6):394-402.

PMID: 24940697

Abstract:

Mycoplasmas are opportunistic parasites and some species are suggested to preferentially colonize tumor tissue in cancer patients. We could demonstrate that the annotated thymidine phosphorylase (TP) gene in the genome of Mycoplasma hyorhinis encodes a pyrimidine nucleoside phosphorylase (PyNPHyor) that not only efficiently catalyzes thymidine but also uridine phosphorolysis. The kinetic characteristics of PyNPHyor-catalyzed nucleoside and nucleoside analogue (NA) phosphorolysis were determined. We demonstrated that the expression of such an enzyme in mycoplasma-infected cell cultures dramatically alters the activity of various anticancer/antiviral NAs such as 5-halogenated pyrimidine nucleosides, including 5-trifluorothymidine (TFT). Due to their close association with human cancers, the presence of mycoplasmas may markedly influence the therapeutic efficiency of nucleoside-based drugs.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9055350	Pyrimidine nucleoside phosphorylase		9055-35-0	Price

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