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The role of arachidonic acid/cyclooxygenase cascade, phosphodiesterase IV and Rho-kinase in H 2 S-induced relaxation in the mouse corpus cavernosum

The role of arachidonic acid/cyclooxygenase cascade, phosphodiesterase IV and Rho-kinase in H 2 S-induced relaxation in the mouse corpus cavernosum

Fatma Aydinoglu, Nuran Ogulener

Pharmacol Rep. 2017 Aug;69(4):610-615.

PMID: 28501682

Abstract:

Background:
Penile corpus cavernosum is an extremely vascularized tissue and cavernosal smooth muscle tone is regulated by the balance between contractile and relaxant factor. We investigated the possible role of arachidonic acid/cyclooxygenase cascade, phosphodiesterase IV (PDEIV) and Rho-kinase in exogenous hydrogen sulfide (H2S)-induced relaxation in mouse corpus cavernosum.
Methods:
The relaxant response to H2S (NaHS as exogenous H2S; 1-1000μM) were obtained in isolated mouse corpus cavernosum tissues which pre-contracted by phenylephrine (5μM). The effects of 4-(4-octadecylphenyl)-4-oxobutenoic acid (OBAA; 10μM), a selective phospholipase A2 (PLA2) inhibitor, indomethacin (1μM), a non-selective cyclooxygenase (COX) inhibitor, baicalein (10μM), a lipoxygenase (LOX) inhibitor, and proadifen (10μM), cytochrome P450 inhibitor, on the relaxant responses to H2S were investigated. Furthermore, the effects of theophylline (500μM) and rolipram (1μM), a non-selective and selective PDEIV inhibitor, and fasudil (3μM), a specific Rho-kinase inhibitor, were studied on H2S-induced relaxation.
Results:
H2S-induced relaxations were significantly reduced by OBAA, indomethacin and proadifen but not baicalein. Furthermore, theophylline, rolipram and fasudil reduced H2S-induced relaxations.
Conclusion:
These results suggest that PLA2, COX, cytochrome P450, PDEIV and Rho-kinase pathway may involve in H2S-induced relaxation in mouse corpus cavernosum tissues.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP134531423	OBAA		134531-42-3	Price

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