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The Trophic Effect of Ouabain on Retinal Ganglion Cell Is Mediated by EGF Receptor and PKC Delta Activation

The Trophic Effect of Ouabain on Retinal Ganglion Cell Is Mediated by EGF Receptor and PKC Delta Activation

Gustavo de Rezende Corrêa, Karinne Cristinne da Silva Cunha, Aline Araujo dos Santos, Elizabeth Giestal de Araujo

Neurochem Res. 2010 Sep;35(9):1343-52.

PMID: 20499167

Abstract:

It was already shown that ouabain treatment can stimulate PKC isoenzymes leading to the activation of intracellular pathways involved in cell survival, growth and proliferation. We have previously demonstrated that ouabain or PMA treatment increases retinal ganglion cell survival, an effect mediated by PKC activation. The aim of this work was to investigate the role of EGF receptors in the ouabain effect and also to study which PKC isoform is activated by treatment with ouabain and PMA. Our results show that 2.5 microM tyrphostin, 1.0 microM PP1, 4.0 microM U73122, 1.0 microM JNK inhibitor V and 2.0 microM rottlerin blocked the ouabain effect indicating an involvement of receptors for EGF, Src, PLC, JNK and PKC delta respectively. The effect of PMA was only abolished when cultures were treated with rottlerin or with the JNK inhibitor suggesting the involvement of PKC delta and JNK. These results indicate that PKC delta could be a key regulator of retinal ganglion cell survival.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2826268	Tyrphostin 1		2826-26-8	Price

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