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The Use of Carotenoids and Oxonol VI as Probes for Membrane Potential in Proteoliposomes

The Use of Carotenoids and Oxonol VI as Probes for Membrane Potential in Proteoliposomes

H S van Walraven, K Krab, M J Hagendoorn, R Kraayenhof

FEBS Lett. 1985 May 6;184(1):96-9.

PMID: 3157600

Abstract:

Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP64724750	Oxonol VI		64724-75-0	Price

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