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The Use of Steroid Profiling in the Resolution of Pregnenolone Metabolites From Porcine Testicular Preparations

The Use of Steroid Profiling in the Resolution of Pregnenolone Metabolites From Porcine Testicular Preparations

T K Kwan, N F Taylor, D B Gower

J Chromatogr. 1984 Sep 28;301(1):189-97.

PMID: 6501488

Abstract:

The behaviour of 22 steroids has been examined by capillary gas chromatographic profiling, and the identities of the steroids confirmed by mass spectrometry and their relative retention times. Five pairs of steroids have been separated: 5 alpha-androstane-3 alpha,17 beta- and -3 beta,17 beta-diols; 5-androstene-3 beta,17 alpha- and -3 beta,17 beta-diols; pregnenolone and 3 beta-hydroxy-5,16-pregnadien-20-one; progesterone and 4,16-pregnadiene-3,20-dione; 17-hydroxypregnenolone and 5-pregnene-3 beta,20 beta-diol. 5 alpha-Androst-16-en-3 beta-ol and 5,16-androstadien-3 beta-ol (as trimethylsilyl-, tert.-butyl-dimethylsilyl- and chloromethyldimethylsilyl ethers) were only partially resolved but could be well separated on thin-layer plates of Kieselgel that had previously been dipped in AgNO3 solution. The profiling method was successfully applied to the separation of pregnenolone and its metabolites in porcine testicular incubation extracts.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP327048862-A	Androstene-3,17-dione-2,3,4-13C3 solution		327048-86-2	Price

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