Home
Resources
Publications
Theaflavins Enhance Intestinal Barrier of Caco-2 Cell Monolayers Through the Expression of AMP-activated Protein Kinase-Mediated Occludin, Claudin-1, and ZO-1

Theaflavins Enhance Intestinal Barrier of Caco-2 Cell Monolayers Through the Expression of AMP-activated Protein Kinase-Mediated Occludin, Claudin-1, and ZO-1

Ha-Young Park, Yuri Kunitake, Naoto Hirasaki, Mitsuru Tanaka, Toshiro Matsui

Biosci Biotechnol Biochem. 2015;79(1):130-7.

PMID: 25175351

Abstract:

We investigated the effect of theaflavins (TFs) on membrane barrier of Caco-2 cells. For fluorescein-transport experiments, the apparent permeability (Papp) of fluorescein in Caco-2 cells pretreated with 20 μM TFs were significantly decreased compared with that in untreated cells. Although the respective monomeric catechins did not show any Papp reduction, purpurogallin pretreatment resulted in a significant Papp reduction similar to that of TF-3'-O-gallate (TF3'G) pretreatment. This indicates that the benzotropolone moiety may play a crucial role in the Papp reduction or tight junction (TJ)-closing effect induced by TFs. In TF-3'-O-gallate-pretreated Caco-2 cells, fluorescein transport was completely restored by compound C (AMPK inhibitor). In addition, TF3'G significantly increased both the mRNA and protein expression of TJ-related proteins (occludin, claudin-1, and ZO-1) as well as the phosphorylation of AMPK. It was, thus, concluded that TFs could enhance intestinal barrier function by increasing the expression of TJ-related proteins through the activation of AMPK in Caco-2 cells.

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: