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Thermo- And pH-Responsive, Coacervate-Forming Hyperbranched Poly(β-amino Ester)s for Selective Cell Binding

Thermo- And pH-Responsive, Coacervate-Forming Hyperbranched Poly(β-amino Ester)s for Selective Cell Binding

Dezhong Zhou, Luca Pierucci, Yongsheng Gao, Jonathan O'Keeffe Ahern, Xiaobei Huang, A Sigen, Wenxin Wang

ACS Appl Mater Interfaces. 2017 Feb 22;9(7):5793-5802.

PMID: 28170215

Abstract:

We report a new type of thermo- and pH-responsive, coacervate-forming highly degradable polymer-hyperbranched poly(β-amino esters) (HPAEs) and its selective cell binding behaviors. The HPAEs were synthesized from 5-amino-1-pentanol (S5) and trimethylolpropane ethoxylate triacrylate (TMPETA) via an A2+B3 type Michael addition. The existence of multiple hydrogen bond pairs as well as tertiary amines makes the S5-TMPETA polymers manifest temperature- and pH-dependent phase transition. By varying the length of the ethylene glycol (EG) spacers in the TMPETA, polymer molecular weight, concentration, and pH value, the phase transition of the S5-TMPETA can be easily tuned in aqueous and buffer solutions, as evidenced by UV-vis spectroscopy and DLS measurements. Especially, the S5-TMPETA prepared from S5 and trimethylolpropane ethoxylate triacrylate 692 (S5-TMPETA692) shows a lower critical solution temperature (LCST) around 33 °C, above which the S5-TMPTEA can form coacervate particles able to encapsulate functional molecules effectively. Importantly, when incubation with HeLa cells, the S5-TMPTETA692 exhibits a temperature- and pH-responsive selective cell binding behaviors. In addition, the S5-TMPETA are highly hydrolyzable and elicit negligible cytotoxicity. This new type of "smart" polymer should find use in a variety of biomedical applications.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP50586599	Trimethylolpropane ethoxylate		50586-59-9	Price

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