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Thin-layer Chromatographic Detection of Glucocorticoids

S M Wu, S H Chen, H L Wu

Gaoxiong Yi Xue Ke Xue Za Zhi. 1991 Nov;7(11):545-9.

PMID: 1766024

Abstract:

An adsorption thin-layer chromatography (TLC) is described for the detection of glucocorticoids including triamcinolone, prednisolone, 6 alpha-methylprednisolone, betamethasone, dexamethasone, hydrocortisone, prednisone and cortisone. Separation of the glucocorticoids was performed on a silica stationary phase with chloroform-dioxane-methanol (15:4:1, v/v) as a mobile phase. The complete resolution of the epimers of betamethasone and dexamethasone was attained by derivatization of them with (1S)-(-)-camphanic chloride using triethylamine as a catalyst. The application of the method to the analysis of glucocorticoids in various samples is being smoothly developed.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP39637746 (1S)-(−)-Camphanic chloride (1S)-(−)-Camphanic chloride 39637-74-6 Price
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