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Three-dimensional Cellular Imaging in Thick Biological Tissue With Confocal Detection of One-Photon Fluorescence in the Near-Infrared II Window

Three-dimensional Cellular Imaging in Thick Biological Tissue With Confocal Detection of One-Photon Fluorescence in the Near-Infrared II Window

Menghan Wang, Nanguang Chen

J Biophotonics. 2019 Jul;12(7):e201800459.

PMID: 30663282

Abstract:

Fluorescence imaging in the second near-infrared optical window (NIR-II, 900-1700 nm) has become a technique of choice for noninvasive in vivo imaging in recent years. Greater penetration depths with high spatial resolution and low background can be achieved with this NIR-II window, owing to low autofluorescence within this optical range and reduced scattering of long wavelength photons. Here, we present a novel design of confocal laser scanning microscope tailored for imaging in the NIR-II window. We showcase the outstanding penetration depth of our confocal setup with a series of imaging experiments. HeLa cells labeled with PbS quantum dots with a peak emission wavelength of 1276 nm can be visualized through a 3.5-mm-thick layer of scattering medium, which is a 0.8% Lipofundin solution. A commercially available organic dye IR-1061 (emission peak at 1132 nm), in its native form, is used for the first time, as a NIR-II fluorescence label in cellular imaging. Our confocal setup is capable of capturing optically sectioned images of IR-1061 labeled chondrocytes in fixed animal cartilage at a depth up to 800 μm, with a superb spatial resolution of around 2 μm.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP155614010	IR-1061		155614-01-0	Price

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