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Thrombospondins: Purification of Human Platelet thrombospondin-1

Thrombospondins: Purification of Human Platelet thrombospondin-1

John M Sipes, Joanne E Murphy-Ullrich, David D Roberts

Methods Cell Biol. 2018;143:347-369.

PMID: 29310787

Abstract:

Thrombospondins are a family of five secreted proteins that have diverse roles in modulating cellular function. Thrombospondins-1 and 2 were identified as matricellular proteins based on their functional roles combined with their transient appearance or accumulation in extracellular matrix at specific times during development and in response to injury or stress in mature tissues. Thrombospondin-1 is a major component of platelet α-granules, which provides a convenient source for purification of the protein. Methods are described to prepare thrombospondin-1 from human platelets in a biologically active form with minimal degradation or contamination with other platelet proteins. A nondenaturing method is described for removing bound transforming growth factor-β1.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414468	Thrombospondin human			Price
IAR4248685	Transforming Growth Factor-β1 from human platelets			Price

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