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Timosaponin B-II Inhibits Pro-Inflammatory Cytokine Induction by Lipopolysaccharide in BV2 Cells

Timosaponin B-II Inhibits Pro-Inflammatory Cytokine Induction by Lipopolysaccharide in BV2 Cells

Wen-Quan Lu, Yan Qiu, Tie-Jun Li, Xia Tao, Lian-Na Sun, Wan-Sheng Chen

Arch Pharm Res. 2009 Sep;32(9):1301-8.

PMID: 19784587

Abstract:

It was reported that the total polysaccharides extracts from Anemarrhenae asphodeloides Bge (Liliaceae, rhizome) could inhibit inflammatory responses in various models. In the present study, the effects of Timosaponin B-II, a purified extract from A. asphodeloidesb, on the expressions of IL-1beta, TNF-alpha and IL-6, the activity of NF-kappaB and the activation of signal pathway related to NF-kappaB were explored in vitro. Timosaponin B-II significantly attenuated increase of these cytokines on both mRNA and protein levels from LPS-stimulated BV2 cells in a dose-dependent manner. The reporter gene assay also showed that the activation of NF-kappaB induced by LPS was inhibited by pre-treatment with Timosaponin B-II. Moreover, western blot results showed that the activation of p38, JNK and P65 had been decreased. These results suggest that both NF-kappaB signal pathway and MAPK pathway were involved in the inhibitory effects of Timosaponin B-II on the expression of pro-inflammatory cytokines.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP136656070	Timosaponin B II		136656-07-0	Price

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