Home
Resources
Publications
Tracing and Separating Plasma Components Causing Matrix Effects in Hydrophilic Interaction Chromatography-Electrospray Ionization Mass Spectrometry

Tracing and Separating Plasma Components Causing Matrix Effects in Hydrophilic Interaction Chromatography-Electrospray Ionization Mass Spectrometry

Anja Ekdahl, Maria C Johansson, Martin Ahnoff

J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Apr 1;923-924:83-91.

PMID: 23500351

Abstract:

Matrix effects on electrospray ionization were investigated for plasma samples analysed by hydrophilic interaction chromatography (HILIC) in gradient elution mode, and HILIC columns of different chemistries were tested for separation of plasma components and model analytes. By combining mass spectral data with post-column infusion traces, the following components of protein-precipitated plasma were identified and found to have significant effect on ionization: urea, creatinine, phosphocholine, lysophosphocholine, sphingomyelin, sodium ion, chloride ion, choline and proline betaine. The observed effect on ionization was both matrix-component and analyte dependent. The separation of identified plasma components and model analytes on eight columns was compared, using pair-wise linear correlation analysis and principal component analysis (PCA). Large changes in selectivity could be obtained by change of column, while smaller changes were seen when the mobile phase buffer was changed from ammonium formate pH 3.0 to ammonium acetate pH 4.5. While results from PCA and linear correlation analysis were largely in accord, linear correlation analysis was judged to be more straight-forward in terms of conduction and interpretation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP4136372	Stachydrine chloride		4136-37-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: