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Tracking the Pathway of Calcium phosphate/DNA Nanoparticles During Cell Transfection by Incorporation of Red-Fluorescing Tetramethylrhodamine Isothiocyanate-Bovine Serum Albumin Into These Nanoparticles

Tracking the Pathway of Calcium phosphate/DNA Nanoparticles During Cell Transfection by Incorporation of Red-Fluorescing Tetramethylrhodamine Isothiocyanate-Bovine Serum Albumin Into These Nanoparticles

Viktoriya Sokolova, Anna Kovtun, Rolf Heumann, Matthias Epple

J Biol Inorg Chem. 2007 Feb;12(2):174-9.

PMID: 17031704

Abstract:

Calcium phosphate nanoparticles were prepared by precipitation from water and were then functionalized by DNA. These particles are taken up by living cells and function as gene transfer agents, i.e., the DNA is brought into a cell's nucleus and is incorporated there into the cell's genome (transfection). DNA which encodes for enhanced green fluorescent protein leads to green fluorescence of successfully transfected cells. By adding the red-fluorescing marker tetramethylrhodamine isothiocyanate-bovine serum albumin (TRITC-BSA) to the nanoparticles, their pathway into the cell and within the cell could be followed by fluorescence microscopy. A clear correlation between the uptake of nanoparticles and the efficiency of transfection was found. Aggregates of DNA/TRITC-BSA alone were not able to enter the cells, i.e., the inorganic nanoparticles are necessary as a carrier through the cell membrane.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR424155	Albumin, Tetramethylrhodamine isothiocyanate bovine			Price

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