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Transforming Growth Factor Beta 1 Increases Collagen Content, and Stimulates Procollagen I and Tissue Inhibitor of metalloproteinase-1 Production of Dental Pulp Cells: Role of MEK/ERK and Activin Receptor-Like kinase-5/Smad Signaling

Transforming Growth Factor Beta 1 Increases Collagen Content, and Stimulates Procollagen I and Tissue Inhibitor of metalloproteinase-1 Production of Dental Pulp Cells: Role of MEK/ERK and Activin Receptor-Like kinase-5/Smad Signaling

Po-Shuen Lin, Hsiao-Hua Chang, Chien-Yang Yeh, Mei-Chi Chang, Chiu-Po Chan, Han-Yueh Kuo, Hsin-Cheng Liu, Wan-Chuen Liao, Po-Yuan Jeng, Sin-Yuet Yeung, Jiiang-Huei Jeng

J Formos Med Assoc. 2017 May;116(5):351-358.

PMID: 27720345

Abstract:

Background/purpose:
In order to clarify the role of transforming growth factor beta 1 (TGF-β1) in pulp repair/regeneration responses, we investigated the differential signaling pathways responsible for the effects of TGF-β1 on collagen turnover, matrix metalloproteinase-3 (MMP-3), and tissue inhibitor of metalloproteinase-1 (TIMP-1) production in human dental pulp cells.
Methods:
Pulp cells were exposed to TGF-β1 with/without pretreatment and coincubation by 1,4-diamino-2,3-dicyano-1,4-bis(o-aminophenyl mercapto)butadiene (U0126; a mitogen-activated protein kinase kinase [MEK]/extracellular signal-regulated kinase [ERK] inhibitor) and 4-(5-benzol[1,3]dioxol-5-yl-4-pyrldin-2-yl-1H- imidazol-2-yl)-benzamide hydrate (SB431542; an activin receptor-like kinase-5/Smad signaling inhibitor). Sircol collagen assay was used to measure cellular collagen content. Culture medium procollagen I, TIMP-1, and MMP-3 levels were determined by enzyme-linked immunosorbent assay.
Results:
TGF-β1 increased the collagen content, procollagen I, and TIMP-1 production, but slightly decreased MMP-3 production of pulp cells. SB431542 and U0126 prevented the TGF-β1-induced increase of collagen content and TIMP-1 production of dental pulp cells.
Conclusion:
These results indicate that TGF-β1 may be involved in the healing/regeneration processes of dental pulp in response to injury by stimulation of collagen and TIMP-1 production. These events are associated with activin receptor-like kinase-5/Smad2/3 and MEK/ERK signaling.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
ALP301836419	SB 431542 hydrate		301836-41-9 (anhydrous)	Price

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