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Transposon Tn5-259 Mutagenesis of Pseudomonas Cepacia to Isolate Mutants Deficient in Antifungal Activity

Transposon Tn5-259 Mutagenesis of Pseudomonas Cepacia to Isolate Mutants Deficient in Antifungal Activity

R K Jayaswal, M A Fernandez, L Visintin, R S Upadhyay

Can J Microbiol. 1992 Apr;38(4):309-12.

PMID: 1377094

Abstract:

Transposon Tn5-259 was inserted into the chromosome of Pseudomonas cepacia by mating with an Escherichia coli strain harboring a self-mobilizable, temperature-sensitive plasmid, pME12. Data from Southern blots and auxotroph analyses indicated that a single copy of the transposon was inserted in several places into the chromosome of P. cepacia. Among 1500 Tn5-259 transconjugants, only one mutant was found to be defective in the production of an antifungal compound, pyrrolnitrin. In addition, this mutant lost its ability to antagonize fungal phytopathogens. Using flanking DNA of the mutated gene as a probe, we have isolated four overlapping cosmid clones from a genomic library of P. cepacia. However, we were unable to complement the mutant because of difficulty in mobilizing the cosmids from E. coli to P. cepacia.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4244513	Pyrrolnitrin from Pseudomonas cepacia			Price

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