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Tributyltin Is a Potent Inducer of the Heat Shock Response in Human Diploid Fibroblasts

Tributyltin Is a Potent Inducer of the Heat Shock Response in Human Diploid Fibroblasts

H Zhang, A Y Liu

J Cell Physiol. 1992 Dec;153(3):460-6.

PMID: 1447308

Abstract:

Submicromolar concentrations of tributyltin (TBT), a commercially used organotin compound, were found to induce the expression of several stress proteins, most notably HSP89 and HSP70, in IMR-90 human diploid fibroblasts in a time- and dose-dependent manner. This induction can be demonstrated by quantitation of 1) synthesis of the heat shock proteins (HSPs), 2) relative abundance of mRNA of hsp70, and 3) transient expression of a human hsp70 promoter driven reporter gene. TBT also increased the abundance of mRNA of heme oxygenase, whereas heat shock was without effect. Analysis of protein binding to a consensus heat shock element (HSE) by electrophoretic mobility shift assay suggests that the induction of the heat shock response by TBT was attributable to activation of the heat shock transcription factor (HSTF).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4245680	Heat Shock Protein 90 human			Price

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