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Tricine-SDS-PAGE

Syed R Haider, Helen J Reid, Barry L Sharp

Methods Mol Biol. 2019;1855:151-160.

PMID: 30426416

Abstract:

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) is an efficient way of separating low molecular mass proteins. However, the standard system is quite complicated and specifically may not be useful when the separated proteins are to be recovered from the gel for quantitative analysis. Here, we describe a simplified system whereby these smaller proteins can be resolved in comparatively low percentage gels which have high compatibility with modern detectors such as UV and inductively coupled plasma-mass spectrometry (ICP-MS).

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9048468-B Protein standard Protein standard 9048-46-8 Price
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