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Tris(hydroxymethyl)aminomethane-functionalized Silica Particles and Their Application for Hydrophilic Interaction Chromatography

Tris(hydroxymethyl)aminomethane-functionalized Silica Particles and Their Application for Hydrophilic Interaction Chromatography

Thi Nhat Hong Bui, Jeroen J Verhage, Knut Irgum

J Sep Sci. 2010 Oct;33(19):2965-76.

PMID: 21038457

Abstract:

A new method is presented for synthesizing a highly hydrophilic silica-based material for use in hydrophilic interaction chromatography. Porous silica particles used as a starting substrate were modified with 3-bromopropyl trichlorosilane and grafted with glycidyl methacrylate by controlled ("living") atom transfer radical polymerization in order to introduce an oxirane-carrying reactive tentacle layer on the silica surface. The grafted material was thereafter subject to an oxirane ring opening reaction with tris(hydroxy-methyl)aminomethane in dimethylformamide to yield a polymer-bound equivalent of the well known and highly hydrophilic "TRIS" buffering substance. Chemical characterization was done by diffuse reflectance FT-IR, X-ray photoelectron spectroscopy, elemental analysis, and (1)H NMR. Porosity and surface area examination was done with Brunauer-Emmett-Teller. Chromatographic application of the material was evaluated by separations of nucleic bases, small organic acids, and common nucleotides under mixed hydrophilic interaction chromatography and weak anion exchange conditions.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP13883391	(3-Bromopropyl)trichlorosilane		13883-39-1	Price

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