Home
Resources
Publications
Tumor Necrosis Factor Alpha Induces Proteins That Bind Specifically to Kappa B-like Enhancer Elements and Regulate Interleukin 2 Receptor Alpha-Chain Gene Expression in Primary Human T Lymphocytes

Tumor Necrosis Factor Alpha Induces Proteins That Bind Specifically to Kappa B-like Enhancer Elements and Regulate Interleukin 2 Receptor Alpha-Chain Gene Expression in Primary Human T Lymphocytes

J W Lowenthal, D W Ballard, E Böhnlein, W C Greene

Proc Natl Acad Sci U S A. 1989 Apr;86(7):2331-5.

PMID: 2494663

Abstract:

We have investigated the biochemical basis for the activation of interleukin 2 receptor alpha-subunit (IL-2R alpha) gene expression in primary human T lymphocytes by a cytokine (tumor necrosis factor alpha), a T-cell mitogen (phorbol 12-myristate 13-acetate), and the transactivator protein (Tax) from the type I human T-cell leukemia virus. Using in vivo transfection techniques specificially designed for these primary T cells in conjunction with in vitro gel retardation and DNA footprinting assays, we found that activation of the IL-2R alpha promoter by each of these agents involves the induction of nuclear proteins that specifically interact with a kappa B-like enhancer element (i.e., an element resembling the immunoglobulin kappa-chain enhancer sequence recognized by transcription factor NF-kappa B). DNA-protein crosslinking studies revealed that primary T cells express at least three different inducible DNA-binding proteins (50-55, 70-75, and 80-90 kDa) that specifically interact with this IL-2R alpha kappa B element.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248883	sTNF-receptor Type I human			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: