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UHPLC-MS/MS Determination and Pharmacokinetic Study of Plantamajoside in Rat Plasma After Oral Administration of Single Plantamajoside and Plantago Asiatica Extract

UHPLC-MS/MS Determination and Pharmacokinetic Study of Plantamajoside in Rat Plasma After Oral Administration of Single Plantamajoside and Plantago Asiatica Extract

Lizhi Bai, Li Han, Xiaoguang Lu, Xin Kang, Zhiwei Fan, Rong Xing, Dangxia Zhou

Biomed Chromatogr. 2017 May;31(5).

PMID: 27808441

Abstract:

A sensitive and reliable ultra-high performance liquid chromatography coupled with tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for quantitation of plantamajoside in rat plasma. First, this study compared the pharmacokinetic properties of plantamajoside after oral administration of Plantago asiatica extract and pure plantamajoside in rat plasma with approximately the same dosage of 8.98 mg/kg. Second, chromatographic separation was performed on an Acquity HSS C18 column (50 × 2.1 mm, p.d.1.7 μm) with isocratic elution using methanol-water (80:20, v/v) as mobile phase at a flow rate of 0.25 mL/min. The calibration curves were linear over the range of 0.1-100 ng/mL for plantamajoside. At different time points (0, 0.083, 0.167, 0.25, 0.5, 0.75, 1, 2, 3, 4, 5, 6 and 8 h) after administration, the concentrations of plantamajoside in plasma were measured and the main pharmacokinetic parameters were estimated. The study indicates that the pharmacokinetics of plantamajoside in rat plasma have significant differences between two groups.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP104777686	Plantamajoside		104777-68-6	Price

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