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UHPLC-MS/MS Quantification Combined With Chemometrics for the Comparative Analysis of Different Batches of Raw and Wine-Processed Dipsacus Asper

UHPLC-MS/MS Quantification Combined With Chemometrics for the Comparative Analysis of Different Batches of Raw and Wine-Processed Dipsacus Asper

Yi Tao, Yingshan Du, Dandan Su, Weidong Li, Baochang Cai

J Sep Sci. 2017 Apr;40(8):1686-1693.

PMID: 28218445

Abstract:

A rapid and sensitive ultra-high performance liquid chromatography with tandem mass spectrometry approach was established for the simultaneous determination of 4-caffeoylquinic acid, loganic acid, chlorogenic acid, loganin, 3,5-dicaffeoylquinic acid, dipsacoside B, asperosaponin VI, and sweroside in raw and wine-processed Dipsacus asper. Chloramphenicol and glycyrrhetinic acid were employed as internal standards. The proposed approach was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery. Intra- and interassay variability for all analytes were 2.8-4.9 and 1.7-4.8%, respectively. The standard addition method determined recovery rates for each analytes (96.8-104.6%). In addition, the developed approach was applied to 20 batches of raw and wine-processed samples of Dipsacus asper. Principle component analysis and partial least squares-discriminate analysis revealed a clear separation between the raw group and wine-processed group. After wine-processing, the contents of loganic acid, chlorogenic acid, dipsacoside B, and asperosaponin VI were upregulated, while the contents of 3,5-dicaffeoylquinic acid, 4-caffeoylquinic acid, loganin, and sweroside were downregulated. Our results demonstrated that ultra-high performance liquid chromatography with tandem mass spectrometry quantification combined with chemometrics is a viable method for quality evaluation of the raw Dipsacus asper and its wine-processed products.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP33289859	Dipsacoside B		33289-85-9	Price

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