Home
Resources
Publications
Ultra-performance Liquid Chromatographic Determination of L-ergothioneine in Commercially Available Classes of Cow Milk

Ultra-performance Liquid Chromatographic Determination of L-ergothioneine in Commercially Available Classes of Cow Milk

Salvatore Sotgia, Elisabetta Pisanu, Debora Cambedda, Gianfranco Pintus, Ciriaco Carru, Angelo Zinellu

J Food Sci. 2014 Sep;79(9):C1683-7.

PMID: 25154712

Abstract:

A new efficient and sensitive precolumn hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) method was established for the quantitative determination of L-ergothioneine (ERT) in milk. After derivatization of ERT with 7-diethylamino-3-[4-(iodoacetamido)phenyl]-4-methylcoumarin, chromatographic separation was achieved in a fairly short time, less than 5 min, on a 100 × 2.1 mm Waters Cortecs UPLC HILIC 1.6-μm column, by using a mixture of 30 mmol/L ammonium acetate/acetonitrile (10:90, v/v) as a mobile phase flowing isocratically at 0.9 mL/min. Limit of detection and the limit of quantification were 0.03 and 0.10 μmol/L, respectively. The method exhibited linearity in a concentration range of 0.16 and 5.08 μmol/L. Mean recovery was 106.66%, whereas intra- and interassay precisions were determined to be within 6 RSD%. On average, ERT concentration in different commercially available classes of cow milk was found to be 0.442 ± 0.191 μmol/L, with the highest levels in the ultra-high temperature milks and low values in the unprocessed and HTST whole milks. In this light, our experiments suggest that ERT could be used as a marker for the heat treatment of milk.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP76877344	7-Diethylamino-3-[4-(iodoacetamido)phenyl]-4-methylcoumarin		76877-34-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: