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Ultrafast Determination of Vitamin E Using LC-ESI-MS/MS for Preclinical Development of New Nutraceutical Formulations

Ultrafast Determination of Vitamin E Using LC-ESI-MS/MS for Preclinical Development of New Nutraceutical Formulations

Noemi Villaseca-González, Virginia Rodríguez Robledo, Lucía Castro-Vázquez, María Victoria Lozano, Manuel J Santander-Ortega, Joaquín González-Fuentes, Pilar Marcos, María Del Mar Arroyo-Jiménez

Bioanalysis. 2018 Feb;10(4):215-227.

PMID: 29333875

Abstract:

Aim:
We proposed a rapid and high quality method to determine α-tocopherol (α-T) in different biopharmaceutical samples using liquid chromatography-diode array detector on-line ESI-MS/MS.
Materials & methods:
A working standard solution of α-T and internal standard, phenyl-5,7-dimethyl-d6-α-tocopherol, were used for optimization and validation of the method. Levels of α-T in nanoemulsions, serum and plasma samples were evaluated.
Results & conclusion:
Precision (1% for retention time, 5% for peak area and 3% for relative peak area), linearity range (among 0.625-20.0 μg ml-1), LOD and LOQ, accuracy and matrix effect were studied. The validated chromatographic method is presented as valuable analytical tool for the determination of α-tocopherol in loaded drug delivery systems and in biodistribution levels in blood samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP10191410-A	(±)−α−Tocopherol (Vitamin E) solution	10191-41-0	Price
AP113892083-B	α-Tocopherol (phenyl-5,7-dimethyl-d6)	113892-08-3	Price
AP96740328	Parathion-methyl-d6 (dimethyl-d6)	96740-32-8	Price

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