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Ultrasensitive Fluorescence Detection of Sequence-Specific DNA via Labeling Hairpin DNA Probes for Fluorescein O-Acrylate Polymers

Ultrasensitive Fluorescence Detection of Sequence-Specific DNA via Labeling Hairpin DNA Probes for Fluorescein O-Acrylate Polymers

Xinxiu Yang, Qianrui Liu, Dongxiao Wen, Meng Gao, Dongjian Zhang, Qiaomei Jin, Jinming Kong, Jian Zhang

Anal Chim Acta. 2019 Dec 11;1088:144-149.

PMID: 31623710

Abstract:

Sensitive detection of DNA is conducive to enhance the accuracy of diseases diagnosis and risk prediction. In this work, we report the use of activators generated by electron transfer for atom transfer radical polymerization (AGET ATRP) as a novel on-chip amplification strategy for the fluorescence detection of DNA. More specifically, the target DNA was captured by the on-chip immobilized hairpin DNA probes. Upon hybridization, exposed 3'-N3 of the hairpin was used to attach AGET ATRP initiators onto the silicon surface by click chemistry. Then, numerous fluorescent labeling linked to the end of the probes via the formation of long chain polymers of fluorescein o-acrylate, which in turn amplified the fluorescence signal for DNA detection. Under optimal conditions, it showed a good linear range from 100 fM to 1 μM in DNA detection, with the limit of detection as low as 4.3 fM. Moreover, this strategy showed good detection performance in complex real serum samples, the fluorescence intensity of 0.1 nM tDNA in 1% fetal bovine serum samples was 97.6% of that in Tris-EDTA buffer. Based on its high sensitivity, reduced cost and simplicity, the proposed signal amplification strategy displays translational potential in clinical application.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP193419862	Fluorescein o-acrylate		193419-86-2	Price

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