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Ultrasound Assisted Enzymatic Hydrolysis for Isolating Titanium Dioxide Nanoparticles From Bivalve Mollusk Before sp-ICP-MS

Ultrasound Assisted Enzymatic Hydrolysis for Isolating Titanium Dioxide Nanoparticles From Bivalve Mollusk Before sp-ICP-MS

María Vanesa Taboada-López, Sara Iglesias-López, Paloma Herbello-Hermelo, Pilar Bermejo-Barrera, Antonio Moreda-Piñeiro

Anal Chim Acta. 2018 Aug 14;1018:16-25.

PMID: 29605130

Abstract:

Applicability of single-particle inductively coupled plasma mass spectrometry (sp-ICP-MS) using dwell times equal to or shorter than 100 μs has been tested for assessing titanium dioxide nanoparticles (TiO2 NPs) in bivalve mollusks. TiO2 NPs isolation from fresh mollusk tissues was achieved by ultrasound assisted enzymatic hydrolysis procedure using a pancreatin/lipase mixture. Optimum extraction conditions imply ultrasonication (60% amplitude) for 10 min, and 7.5 mL of a solution containing 3.0 g L-1of pancreatin and lipase (pH 7.4). The developed method was found to be repeatable (repeatability of 17% for the over-all procedure, TiO2 NPs concentration of 5.33 × 107 ± 8.89 × 106, n = 11), showing a limit of detection of 5.28 × 106 NPs g-1, and a limit of detection in size of 24.4-30.4 nm, based on the 3σ criteria, and on the 3σ/5 σ criteria, respectively. The analytical recovery within the 90-99% range (use of TiO2 NPs standards of 50 nm at 7 and 14 μg L-1 as Ti). Several bivalve mollusks (clams, cockles, mussels, razor clams, oysters and variegated scallops) were analyzed for total titanium (ICP-MS after microwave assisted acid digestion), and for TiO2 NPs by the proposed method. TiO2 NPs concentrations were within the 2.36 × 107-1.25 × 108 NPs g-1 range, and the most frequent sizes were from 50 to 70 nm.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP8049476-B	Pancreatin lipase		8049-47-6	Price

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