Home
Resources
Publications
Ultrastructural Characteristics of Fc Epsilon R-positive Basophils in the Spleen and Bone Marrow of Mice Immunized With Goat Anti-Mouse IgD Antibody

Ultrastructural Characteristics of Fc Epsilon R-positive Basophils in the Spleen and Bone Marrow of Mice Immunized With Goat Anti-Mouse IgD Antibody

A M Dvorak, R A Seder, W E Paul, S Kissell-Rainville, M Plaut, S J Galli

Lab Invest. 1993 Jun;68(6):708-15.

PMID: 8515657

Abstract:

Background:
Previous work showed that injection of mice with goat anti-mouse IgD antibodies results in increased numbers of Fc epsilon R-positive, non-B, non-T cells in the spleen and Fc epsilon R-positive cells in the bone marrow, and that some of these cells had ultrastructural features of basophils. Fc epsilon R-positive, non-B, non-T cells express virtually all of the capacity of mouse splenic "non-B, non-T cells" to produce interleukin-4 in response to stimulation by cross-linking of Fc epsilon R or Fc gamma R, or by the calcium ionophore, ionomycin.
Experimental design:
The present study is a detailed ultrastructural analysis of Fc epsilon R-positive bone marrow cells or Fc epsilon R-positive splenic non-B, non-T cells sorted from mice injected with goat anti-mouse IgD antibody and of Fc epsilon R-positive bone marrow cells or spleen cells pooled from normal mice not injected with goat anti-IgD.
Results:
Basophils represented the majority (90%) of the granulated cells present in the Fc epsilon R-positive splenic non-B, non-T cells or Fc epsilon R-positive bone marrow cells of goat anti-IgD-injected mice. In contrast, the cytoplasmic granule-containing Fc epsilon R-negative cells sorted from spleen or bone marrow of goat anti-IgD-injected animals contained predominantly a mixture of neutrophils, eosinophils, monocytes and their precursors. Both the Fc epsilon R-positive and -negative preparations contained rare (< 5%) cells with ultrastructural features of very immature mast cells. Basophils were also identified in Fc epsilon R-positive cells sorted from total bone marrow cells or spleen cells of normal mice not injected with goat anti-IgD.
Conclusions:
Taken together with data concerning the numbers of Fc epsilon R-positive, non-B, non-T cells in the spleen, and Fc epsilon R-positive B220-negative cells in the bone marrow, these ultrastructural findings indicate that injection of mice with goat anti-IgD results in increased numbers of basophils, particularly in the spleen, that exhibit an 8-fold increase in basophils as a result of injection of goat anti-IgD.

Chemicals Related in the Paper:

Catalog Number	Product Name	Price
IAR42412756	Anti-PERP antibody produced in goat	Price
IAR4246016	Anti-FOXC2 antibody produced in goat	Price
IAR4246025	Anti-MSX1 antibody produced in goat	Price
IAR4246827	Anti-TRPV5 antibody produced in goat	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: