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Uptake of Arachidonic Acid, Arachidic Acid, Oleic Acid and Their Incorporation Into Phospholipids and Triacylglycerols of Isolated Murine Hepatocytes. Effect of Thrombin-Antithrombin III Complex

Uptake of Arachidonic Acid, Arachidic Acid, Oleic Acid and Their Incorporation Into Phospholipids and Triacylglycerols of Isolated Murine Hepatocytes. Effect of Thrombin-Antithrombin III Complex

J Mandl, B Tanács, Z Spolarics, T Garzó, I Mucha, F Antoni, R Machovich, I Horváth

Thromb Res. 1984 Aug 15;35(4):407-14.

PMID: 6435278

Abstract:

Uptake and metabolism of arachidonic acid, arachidic acid and oleic acid were investigated in isolated hepatocytes prepared from mouse liver with the collagenase perfusion method. The rate of uptake of arachidonic acid was time- and concentration- dependent. 94-98% of the arachidonic acid was incorporated into the phospholipid and triacylglycerol fractions following a 60 min incubation period at 37 degrees C. In the presence of thrombin-anti-thrombin III complex a change in the distribution of arachidonic acid incorporated into lipid fractions was found, i.e. increased incorporation into phosphatidyl-serine and phosphatidylethanolamine, whereas the uptake was not altered. There was no change in the uptake and incorporation of arachidic acid and oleic acid.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP506309	Arachidic acid		506-30-9	Price

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