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Use of 4-bromomethyl-7-methoxycoumarin for Derivatization of Pyrimidine Compounds in Serum Analysed by High-Performance Liquid Chromatography With Fluorimetric Detection

Use of 4-bromomethyl-7-methoxycoumarin for Derivatization of Pyrimidine Compounds in Serum Analysed by High-Performance Liquid Chromatography With Fluorimetric Detection

S Yoshida, S Hirose, M Iwamoto

J Chromatogr. 1986 Nov 28;383(1):61-8.

PMID: 3818846

Abstract:

Derivatization of the pyrimidine nucleobases and nucleosides with 4-bromomethyl-7-methoxycoumarin was studied with the aim of developing a sensitive and selective column liquid chromatographic method for these substances in serum. The labeling reactions and the nature of derivatives are discussed, together with the chromatographic properties of these derivatives. The derivatives are stable for at least several weeks. Typical detection limits are 50 pg for inosine, 150 pg for uridine, 50 pg for uracil, 50 pg for thymine and 100 pg for fluorodeoxyuridine, respectively. Within-day coefficients of variation averaged 5.0% for the stored-frozen serum pools; the mean day-to-day value was 5.2%. Thirty samples could be processed per working day.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP35231448	4-Bromomethyl-7-methoxycoumarin		35231-44-8	Price

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