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Use of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside for the Isolation of Beta-Galactosidase-Positive Bacteria From Municipal Water Supplies

Use of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside for the Isolation of Beta-Galactosidase-Positive Bacteria From Municipal Water Supplies

A Ley, S Barr, D Fredenburgh, M Taylor, N Walker

Can J Microbiol. 1993 Sep;39(9):821-5.

PMID: 10755843

Abstract:

A new medium, mX-Gal, has been developed for the membrane filter enumeration of beta-galactosidase-positive bacteria in municipal water supplies. mX-Gal medium contains the chromogenic beta-galactosidase substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). All Aeromonas, Citrobacter, and Enterobacter strains isolated from raw water on mX-Gal medium were beta-galactosidase positive. In contrast, only 10 to 20% of these strains produced a red colony with a metallic sheen on m-Endo agar LES medium. Of 674 chlorinated water samples analyzed for total coliforms on m-Endo agar LES medium and for beta-galactosidase-positive bacteria on mX-Gal medium, 18 that were negative for coliforms on m-Endo agar LES showed beta-galactosidase-positive bacteria on mX-Gal. Of a total of 50 beta-galactosidase-positive bacteria isolated from these samples, 76% were identified as Aeromonas hydrophila.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7240906	5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside		7240-90-6	Price

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